16s rrna gene sequencing and pcr amplification lactic acid bacteria pdf

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16s rrna gene sequencing and pcr amplification lactic acid bacteria pdf

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Phenotypic and genotypic characterization of lactic acid bacteria isolated from cow, ewe and goat dairy artisanal farmhouses. Stella M. Reginensi; Marcela J. Lactic acid bacteria collected from artisanal farmhouses were characterized using a polyphasic approach.

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Many Lactobacillus species are frequently isolated from dairy products, animal guts, and the vaginas of healthy women. In this study, we developed a multiplex PCR method to distinguish six closely related species in the Lactobacillus acidophilus group L. Altogether, 86 genomes of 9 Lactobacillus species from the National Center of Biotechnology Information NCBI database were compared to detect species-specific genes and design six species-specific primer sets. A final multiplex PCR condition was also optimized for a mixture of all six primer sets mixed. When identifying a single strain, the optimized multiplex PCR method can specifically detect one of the six species, but no band was amplified at least from the other Lactobacillus and Enterococcus species. These results indicated that species-specific primer sets designed from the genome comparison could identify one strain within the six Lactobacillus species by a single PCR reaction.

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Thank you for visiting nature. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser or turn off compatibility mode in Internet Explorer. In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. In general, with a single-copy gene, there are obviously varied DNA sequences for even the same microbial species, which could cause difficulties with design of primers and probes for PCR when targeting various single copy genes.

Twelve probiotic Lactobacillus strains which were previously identified with classical biochemical tests were re-identified using molecular methods. Results of the study showed that mis-identification at species level occurred at high rate when classical biochemical tests were used. Nine of the strains showed discrepancy in their identity. These nine strains which were previously identified through biochemical tests as L. Download to read the full article text.

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Box 07, Wolkite, Ethiopia. The data that support the findings of this study are available from the corresponding author upon sensible request. Injera is soft fermented baked product, which is commonly prepared from teff Eragrostis tef Zucc. As it is a product of naturally fermented dough, the course of fermentation is done by consortia of microorganisms. The study was aimed at isolating and identifying some dominant bacteria from fermenting teff Eragrostis tef dough.

We describe an efficient, robust, and cost effective method for extracting nucleic acid from swabs for characterization of bacterial communities using 16S rRNA gene amplicon sequencing. The method allows for a common processing approach for multiple sample types and accommodates a number of downstream analytic processes. There is a growing appreciation for the role of microbial communities as critical modulators of human health and disease. High throughput sequencing technologies have allowed for the rapid and efficient characterization of bacterial communities using 16S rRNA gene sequencing from a variety of sources. Although readily available tools for 16S rRNA sequence analysis have standardized computational workflows, sample processing for DNA extraction remains a continued source of variability across studies.

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AbstractS rRNA gene analysis of bacteria lactic acid (LAB) isolate from Mar%​isa Kuning protease enzyme activity and 16S rRNA gene amplification using PCR. Then, 16S rRNA gene amplificatiom and DNA sequencing has been done.